Rob Campbell

Rob Campbell

@raacampbell.com

Advanced Microscopy Facility Manager Sainsbury Wellcome Centre, UCL http://swcmicroscopy.com

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Preprint is out! Our BakingTray software turns a 2-photon microscope into a serial-section tomography system for automated whole-organ imaging. ~€35k to convert an existing scope. 11 systems worldwide, >7,000 samples at SWC alone #neuroskyence #microscopy www.biorxiv.org/content/10.6...

BrainSaw microscope at the Max Planck Institute for Biological Intelligence, Bonhoeffer Lab. The microscope is based around a Sutter MOM with four detection channels.

Preprint is out! Our BakingTray software turns a 2-photon microscope into a serial-section tomography system for automated whole-organ imaging. ~€35k to convert an existing scope. 11 systems worldwide, >7,000 samples at SWC alone #neuroskyence #microscopy www.biorxiv.org/content/10.6...

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Here's a short clip of the acquisition software in action, tile-scanning a four-sample block. Terminal window at top-right is from the stitching & pre-processing PC. BrainSaw has contributed to 35 papers so far. Learn more at: swcmicroscopy.com/brainsaw

Preprint is out! Our BakingTray software turns a 2-photon microscope into a serial-section tomography system for automated whole-organ imaging. ~€35k to convert an existing scope. 11 systems worldwide, >7,000 samples at SWC alone #neuroskyence #microscopy www.biorxiv.org/content/10.6...

BrainSaw images whole brains. We acquire most of our data from 4 µm/pixel for (e.g. for brain-wide projection mapping), down to 1.2 µm/pixel to visualise single axons.

BakingTray plugs into ScanImage via its API. ScanImage handles image acquisition; BakingTray handles the logistics of serial sectioning: tiling, slicing, and finding the tissue. The software is modular and supports a range of hardware and configurations. Docs: bakingtray.swcmicroscopy.com

Rob Campbell reposted

Our BrainSaw serial-section 2-photon platform acquires whole-organ datasets automatically and requires minimal tissue processing. It has a growing international install base and has contributed to 35 publications. Learn more at: swcmicroscopy.com/brainsaw/ #microscopy #neuroscience

Rob Campbell reposted

Remember how power bricks used to be bigger, heavier, hotter, and less powerful than power modern power supplies? The SNES power supply can provide 8.5W and this much smaller GaN USB-PD can supply 65W, at greater efficiency. Well, the same thing is probably going to happen to grid transformers. 🧵

Rob Campbell reposted

The BioImage Interest Group is expanding! London-BIIG is bringing together microscopists and computational scientists across UCL, Crick, KCL and Imperial. Follow us for updates on bioimage analysis talks (@LMCB & Zoom) as well as next year's networking event! 🔬🧫 #L-BIIG

Rob Campbell reposted

Replying to @profbillmcguire.bsky.social

Burham can't control the narrative. There will be thousands of screenings of this plus Q&A all over the country. Let's hope he realises that sooner rather than later otherwise it will get very tedious very quickly www.nebriefing.org

nebriefing.orgNational Emergency Briefing on climate & natureNational Emergency Briefing | The UK’s leading climate and nature experts brief MP's on the risks, impacts and solutions to the climate emergency

Rob Campbell reposted

"A water industry source said these plans were not published to avoid alarming the public. The government said the plans were not published for security reasons" Following Starmer's line, Burnham & Co. refuse to inform the public about what's coming Insane www.theguardian.com/environment/...

theguardian.comUK government ‘irresponsible’ for not publishing emergency drought plansCampaigners demand greater transparency into water companies’ plans not made public due to ‘security reasons’

Rob Campbell reposted

Rob Campbell reposted

Excited to share our work now in Nature Methods! We wrote this manuscript to help others better analyze and interpret calcium imaging data. As a central aspect, we show why nonlinear behavior of calcium indicators matters in practice. Very happy about this work!

Rob Campbell reposted

Very excited about what the new version of BrainGlobe will enable: faster, more reliable data access, more atlases, web-based tools and streamlined development in the future. Read more in the BrainGlobe blog: brainglobe.info/blog/braingl...

brainglobe.infobrainglobe-atlasapi V3 is here! — BrainGlobe
BrainGlobe@brainglobe.info

BrainGlobe Atlas API V3 is out! After 6 years, this is the first major change in how BrainGlobe works. Each atlas component is fetched independently, meaning all BrainGlobe tools will work faster, and more reliably. Read more at: brainglobe.info/blog/braingl...

Rob Campbell reposted

nature.comMethods, trade-offs and opportunities in high-speed optical microscopy for neural voltage imagingAdam, Y. et al. Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics. Nature 569, 413–417 (2019). Villette, V. et al. Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice. Cell 179, 1590–1608 (2019). Fan, L. Z. et al. All-optical physiology resolves a synaptic basis for behavioral timescale plasticity. Cell 186, 543–559 (2023). Wenzel, M., Hamm, J. P., Peterka, D. S. & Yuste, R. Reliable and elastic propagation of cortical seizures in vivo. Cell Rep. 19, 2681–2693 (2017). Sato, T. K., Nauhaus, I. & Carandini, M. Traveling waves in visual cortex. Neuron 75, 218–229 (2012). Zhao, H. T. et al. Neurovascular dynamics of repeated cortical spreading depolarizations after acute brain injury. Cell Rep. 37, 109794 (2021). Zhang, Y. et al. Fast and sensitive GCaMP calcium indicators for imaging neural populations. Nature 615, 884–891 (2023). Manley, J. et al. Simultaneous, cortex-wide dynamics of up to 1 million neurons reveal unbounded scaling of dimensionality with neuron number. Neuron 112, 1694–1709 (2024). Yang, Y., Li, B. & Li, Y. Genetically encoded sensors for the in vivo detection of neurochemical dynamics. Annu. Rev. Anal. Chem. 17, 367–392 (2024). Wei, Z. et al. A comparison of neuronal population dynamics measured with...

Rob Campbell reposted

At Cosyne, Chris Olah from Anthropic told us that neuroscientists had “3-4 years left to make a contribution” Why was not clear. Perhaps he was hinting at the below graph accompanying today’s Claude Science launch. Apparently we’ll all be obsolete soon.

Rob Campbell reposted

Our new preprint 'NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations' is out! 🎉Check out @guijacquemet.bsky.social post first to learn more🤩 In this 🧵 I’ll share how we used NucleiSky in smart microscopy and how you can install and try it yourself 👇

Guillaume Jacquemet@guijacquemet.bsky.social

Delighted to share our latest preprint "NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations" www.biorxiv.org/content/10.6... Code and App: github.com/CellMigratio...

Rob Campbell reposted

The top frame is from 2015, when French meteorologists did a “forecast” from a #climate heated August 2050 - a warning on what could possibly happen in 35 years time. Bottom frame: well, it’s happened in barely a decade, only it’s already *much* worse than predicted. It’s a runaway train.

Rob Campbell reposted

Since I did do my PhD in cyanobacteria growth and treatment optimization modeling AND work in the water treatment industry, I’m gonna share my theory as to what’s happening here and how to fix it. People are focusing on the painted bottom of the pool increasing residual heat, and while that 1/19

derek guy@dieworkwear.bsky.social

RIP Mark Rothko. You would have loved how the hydrogen peroxide bleach is only turning the Reflecting Pool blue around the perimeter

Rob Campbell reposted

Doing a linear stretch on these images shows how dramatic the "edits" were, revealing the deception in clear terms. This should be a complete scandal for Thermo Fisher and more people in the biosciences should be talking about it.

Reese Richardson@reeserichardson.bsky.social

The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes: 🖌️ Dozens more images with duplications or painting 🖨️ Hundreds of blots that all share the same background (behold slideshow below)

Rob Campbell reposted

Surprised to discover that Thermo Fisher appears to show a fake western blot for the validation of one of their p53 antibodies. I've added a diagram to show the very similar bands. This does not appear to be one of the "published figures" but their own internal data.