Journal of Cell Biology

The papers in our new #Tools collection present methodological advances that make new questions accessible to investigation, and each applies its method to a biological problem, demonstrating the power of the approach while revealing novel biological insights. 👉 rupress.org/jcb/collecti...

Swollen #lysosomes grab Rabs for a CASM-free route to #LRRK2 activation. Suzanne R. Pfeffer @suzannepfeffer.bsky.social @stanfordmedicine.bsky.social highlights new work from Clegg et al. of the @shawnferguson.bsky.social lab (rupress.org/jcb/article/...) in Spotlight: rupress.org/jcb/article/...

I‘d like to thank the editors and reviewers for their constructive feedback, which helped us improve the quality of our work!

ClC-7 links PIKfyve inhibition to Rab-dependent LRRK2 activity at #lysosomes. New study from Devin Clegg, Shawn M. Ferguson @shawnferguson.bsky.social and colleagues @yalecellbiology.bsky.social: rupress.org/jcb/article/... #CellSignaling #Disease #Organelles

Viewpoint: Zhiqi Tian, Yujie Sun, and Jiajie Diao discuss how #mitochondria supply protons to acidify #lysosomes through direct membrane contacts, and consider implications for mitochondrial quality control, aging, and disease therapeutics. rupress.org/jcb/article/... #Biophysics #Organelles

a-catenin phosphorylation is tension sensitive and supports epithelial barrier function via Afadin. New study from Jeanne M. Quinn, Cara J. Gottardi @carajgottardi.bsky.social and colleagues @feinberg.northwestern.edu: rupress.org/jcb/article/... #Cytoskeleton #Mechanobiology #Adhesion #Development

A Biotin-HaloTag ligand enables efficient affinity capture of protein variants from live cells. New tools developed by Anoop Kumar Yadav, Abhijeet Jadhav, @hanasedlackova.bsky.social and colleagues: rupress.org/jcb/article/... 📕 Part of our #Tools collection: rupress.org/jcb/collecti...

“One of the things I like best about being an editor at the JCB is the partnership between professional and academic editors.”— Mark Peifer (@peiferlabunc.bsky.social), University of North Carolina at Chapel Hill. JCB is your partner for peer review. Learn more 👉 hubs.la/Q04zW3kL0

The role of cell growth rate on accumulation of the mitotic #cyclin Cdc13 in #FissionYeast. New report from Sarah E. Vandal, James B. Moseley @moseleylab.bsky.social @dartmouthbcb.bsky.social and colleagues: rupress.org/jcb/article/... #Biochemistry #Genetics #CellCycle #CellDivision

#Drosophila #dendrites are resilient to adult-onset deficits in mitochondrial dynamics. new study from Hailey Q. Wang, Demetria G. Fortson, Eavan D. Burton, Erin L. Barnhart and colleagues @columbiauniversity.bsky.social: rupress.org/jcb/article/... #Neuroscience #Organelles #Mitochondria

A #nematode-trapping #fungus orchestrates #polarity cues, #septins, and NOX signaling for trap formation. New study from Chih-Yen Kuo @chih-yen.bsky.social, Yen-Ping Hsueh @fungi-worms.bsky.social et al. @maxplanck.de @imb-as.bsky.social: rupress.org/jcb/article/... #Microbiology #Cytoskeleton

New Perspective: Gilles Hickson @cra-chusj.bsky.social revives a material-processing view of #cytokinesis, asking how a shrinking membrane–cortex interface sustains closure despite continued material inflow. rupress.org/jcb/article/... #CellCycle #CellDivision #Cytoskeleton

Kaade, Ohkura et al. @edinburgh-uni.bsky.social identify Sle as an integral component of the spindle matrix, & the Gwl kinase as a spindle envelope protein in #Drosophila #oocytes. They work together to ensure proper structure & function of the meiotic spindle in oocytes. rupress.org/jcb/article/...

ARF-1 coordinates cargo sorting at FERARI endosomal recycling hubs. New study from Jachen Armon Solinger, Anne Spang @spanglab.bsky.social @biozentrum.unibas.ch and colleagues: rupress.org/jcb/article/... #Trafficking #Physiology #Organelles #Endosomes

AI-directed voxel extraction and volume EM identify intrusions as sites of mitochondrial contact. From Benjamin S. Padman, Runa S.J. Lindblom, and Michael Lazarou: rupress.org/jcb/article/... 📕 Part of our #Tools collection: rupress.org/jcb/collecti...

Declining #centromere strength compromises meiotic 3chromosome segregation in #aging #oocytes. New study by Tom Lemonnier, Marie Dumont, Stefanie Redemann, Daniele Fachinetti, Binyam Mogessie @binyammogessie.bsky.social @mcdb-yale.bsky.social and colleagues: rupress.org/jcb/article/... #Meiosis

Biogenesis of nuclear #LipidDroplets is regulated by a Sir2-Csa1-Lro1 signaling pathway at the INM. new study from Lauren Carson, Hui Jin, and Hong-Guo Yu (Florida State University): rupress.org/jcb/article/... #CellMetabolism #Genetics #CellSignaling

Check out our paper!! It was such a fun project to work on. Also fantastic mentoring during my master's thesis by Dumont lab members🌟

Check out our new paper in JCB today all about septins!

New work by Faria, Janody et al. @i3suporto.bsky.social reveals that P-#cadherin engagement and FAK activate mechanosensitive proliferation in premalignant Src-activated cells, highlighting a key role for adhesion-mediated signaling before malignant progression. rupress.org/jcb/article/... #Cancer

Using microneedle manipulation, Mullin-Bernstein et al. @dumontlabucsf.bsky.social show that #microtubule bundles of the mammalian #anaphase spindle are mechanically coupled, transmitting force both laterally and between spindle poles. rupress.org/jcb/article/... #Mitosis #CellDivision #Biophysics

PILS-Nir1 is a sensitive phosphatidic acid #biosensor that reveals mechanisms of #lipid production. From Claire Weckerly (@cwecks.bsky.social), Taylor Rahn, @gerryhammond.bsky.social and colleagues: rupress.org/jcb/article/... 📕 Part of our #Tools collection: rupress.org/jcb/collecti...

The effects of pH on cortex and condensate formation. New study from Brooke E. Waechtler, Douglas N. Robinson and colleagues @jhu.edu: rupress.org/jcb/article/... #Biochemistry #Cytoskeleton #Actin

Septin cytoskeleton acts like intermediate filaments! Our & @pwoakes.bsky.social newest papers in @jcb.org show septins relieve mechanical stress, a key oncogenic trait that can help metastatic tumors survive. Exciting translational implications, a new player in mechanobiology, sick imaging!

Cool #TeamTomo tool from the @becklab.bsky.social for quantifying the thickness of native membranes. And a great use of two community resources: 1) The #ChlamyDataset: bsky.app/profile/life... 2) #MemBrain: bsky.app/profile/lore... Thanks for this great tool. This is how #OpenScience works! 🔬🧪

Huizar, Ewald et al. @jhu.edu show that E-#cadherin promotes #BreastCancer cell survival and #metastasis by suppressing mitochondrial ROS. The cytoplasmic domain of E-cadherin is essential, while extracellular domains, and adhesion and catenin interactions are not. rupress.org/jcb/article/...

Systematic membrane thickness variation across cellular #organelles revealed by #cryoET. New computational workflow developed by @deglushk.bsky.social, Stefanie Böhm, and @becklab.bsky.social: rupress.org/jcb/article/... 📕 Part of our #Tools collection: rupress.org/jcb/collecti...

FAPs orchestrate Antxr2-mediated fine-tuning of #collagen VI to ensure #SkeletalMuscle function. New study from Samuele Metti, F. Gisou van der Goot (@gisouvggoot.bsky.social, EPFL), Paolo Bonaldo (University of Padova) and colleagues: rupress.org/jcb/article/... #Physiology #ExtracellularMatrix

Congratulations to Devin Clegg from the Ferguson Lab on his work determining how PIKfyve inhibition leads to LRRK2 activation at lysosomes! @shawnferguson.bsky.social

Jain, Izadi-Seitz and Ji et al. identify mechanisms & cellular functions of the N-Ank protein Ankrd24 in shaping nerve cells by coordinating remodeling of membrane topologies and cytoskeletal structures, shedding light on proper neuronal arbor development. rupress.org/jcb/article/... #Neuroscience

#Microtubules and nuclear pores position #centromeres and genes in neural #StemCells. New study from Jennifer A. Taylor and Clemens Cabernard @uwbiology.bsky.social: rupress.org/jcb/article/... #Chromatin #epigenetics #Drosophila

Surface morphometrics reveals local membrane thickness variation in organellar subcompartments. From @mmedina300kv.bsky.social, @attychang.bsky.social, @baradlab.com, @nanigrotjahn.bsky.social and colleagues: rupress.org/jcb/article/... 📕 Part of our #Tools collection: rupress.org/jcb/collecti...

MapZ phosphorylation drives EzrA recruitment to the future division site of #Streptococcus pneumoniae. New study from Cassandra Lenoir, Hugo Millat, Christophe Grangeasse and colleagues @mmsb-lyon.bsky.social: rupress.org/jcb/article/... #Microbiology #CellCycle #CellDivision

This artwork (rendered from real FIB-SEM data) was super fun to conceptualize and realize in its final form, and I'm honored to have it featured by @jcb.org! Read our paper if you're interested to learn more about the features we discovered in epithelial desmosomes.

Check the video summarizing our last work at @jcb.org @ibv-csic.bsky.social @csic.es #mitosis

Spotlight: Clegg, @shawnferguson.bsky.social et al. (doi.org/10.1083/jcb....) show that PIKfyve inhibition swells #lysosomes, traps Rab #GTPases, and activates #LRRK2 without the GABARAP–CASM pathway. @suzannepfeffer.bsky.social discusses the findings: rupress.org/jcb/article/...

Clegg, @shawnferguson.bsky.social et al. @yalecellbiology.bsky.social show that LRRK2 integrates distinct lysosomal stress signals through stimulus-specific combinations of interactions with Rab GTPases and GABARAP. rupress.org/jcb/article/... #Lysosomes #CellSignaling #Disease #Organelles

Quinn, @carajgottardi.bsky.social et al. @feinberg.northwestern.edu show how force-dependent phosphorylations on the cell–cell adhesive scaffold, α-catenin, drive apical junction enrichment and barrier function, and how loss of this mechanism leads to hydrocephalus. rupress.org/jcb/article/...

Vandal et al. @moseleylab.bsky.social combine experimental and mathematical approaches to define how time-dependent accumulation of fission yeast cyclin Cdc13 occurs through constant synthesis of a stable protein countered by dilution through cell growth. rupress.org/jcb/article/... #CellCycle

In vivo mapping of #peroxisome dynamics and #pexophagy using PO-TRG and CA-PO-TRG reporter mice. From Yue Xiong, Weihua Gao, Zelai Wu, Xiukui Gao, Qiming Sun and colleagues: rupress.org/jcb/article/... 📕 Part of our #Tools collection: rupress.org/jcb/collecti...

Different variants, different cellular outcomes: Balanced #KIF1A activity shapes synapse development. Shinsuke Niwa (Hokkaido University) discusses work from @aikenlab.bsky.social @erikaholzbaur.bsky.social et al. (rupress.org/jcb/article/...) in Spotlight: rupress.org/jcb/article/... #Neuroscience

check out our newest contribution to the understanding on how to efficiently recycle! great work by Jachen Solinger!

A team at the University of Basel has identified a molecular switch that controls how cells ensure that the right cargo ends up in the right recycling vesicle. www.eurekalert.org/news-release... See the original article from @spanglab.bsky.social @biozentrum.unibas.ch: rupress.org/jcb/article/...