Structural Biology Communications

Our September issue is out! journals.iucr.org/f/issues/202... The cover shows the structure of the NKX2-1 homeodomain bound to a 19 bp DNA duplex containing two palindromically arranged NK2-recognition motifs. Read more at doi.org/10.1107/S205...

Our September issue is out! journals.iucr.org/f/issues/202... The cover shows the structure of the NKX2-1 homeodomain bound to a 19 bp DNA duplex containing two palindromically arranged NK2-recognition motifs. Read more at doi.org/10.1107/S205...

WebCalEM is a browser-based tool that makes lattice-based pixel-size calibration a routine step in the cryo-EM workflow #CryoElectronMicroscopy #MagnificationCalibration #PixelSize doi.org/10.1107/S205...

The replacement of DTT with TCEP in the crystallization of human aldehyde oxidase provides a strategy to circumvent enzyme inactivation during crystallographic studies #HumanAldehydeOxidase #XRayCrystallography #TCEP doi.org/10.1107/S205...

The crystal structure of LegY, a putative glycosidase effector from L. pneumophila, was determined to 1.46 Å resolution and is structurally assigned as a member of glycoside hydrolase family 15 #LegionellaPneumophila #BacterialToxin #Glycosidase doi.org/10.1107/S205...

We would like to invite you to share protocols that will be useful to the community, and help maintain Acta Crystallographica Section F and and the other IUCr Journals as important references for how structural biology research is performed doi.org/10.1107/S205...

Thanks for highlighting our work. I hope it helps the community. Let me know if the Protocol works for you and what @cryosparc.bsky.social could improve.

Presenting the first article in our new 'Protocols' category: Helical reconstruction of amyloids in cryoSPARC #Amyloids #CryoEM #HelicalReconstruction doi.org/10.1107/S205...

The crystal structure of human DKK4-CRD1 determined at 1.83 Å resolution reveals a compact fold comprising N- and C-subdomains connected by a linker region and stabilized by five conserved disulfide bonds #WntSignaling #Dickkopf4 #CysteineRichDomain1 doi.org/10.1107/S205...

The DUF2875 domain-containing T6SS adaptor protein Tla3 from Pseudomonas aeruginosa strain UCBPP-PA14 shows a thiolase-like fold, marked by a five-layered α–β–α–β–α core topology #T6SS #Adaptors #PseudomonasAeruginosa doi.org/10.1107/S205...

The cytosolic Nudix-like domain of the CBASS effector EfCap16 from E. faecalis was determined. The characteristic Nudix-like fold supports classification of EfCap16 as a member of the Nudix superfamily #CBASS #NudixHydrolases #TransmembraneEffectors doi.org/10.1107/S205...

August issue is out! journals.iucr.org/f/issues/202... On the cover: the crystal structure of LegY, a putative glycosidase effector from Legionella pneumophila, shows high similarity to fungal glucoamylases but shares lower homology with known prokaryotic counterparts doi.org/10.1107/S205...

August issue is out! journals.iucr.org/f/issues/202... On the cover: the crystal structure of LegY, a putative glycosidase effector from Legionella pneumophila, shows high similarity to fungal glucoamylases but shares lower homology with known prokaryotic counterparts doi.org/10.1107/S205...

Ok, time to feel old – for a good cause 😃 I was in the middle of my PhD when our journal was launched. An online-only journal at a time when most others were not. This is by definition a VERY special issue. Have a look at what papers we're looking for and be a part of the future of the journal!

Some questions for our structural biology community: What protocols do you think would be useful to have? Do you prefer to watch step-by-step videos, or follow written instructions? Of course, not everything has to be a video, but certain workflows are simply best documented on video #protocols

A new type of article for Acta F: Protocols The purpose of this new category is to provide to the reader procedures that are sufficiently detailed so other researchers can reproduce the described workflow. Find out more in 'Show me how it's done: introducing Protocols': doi.org/10.1107/S205...

We're celebrating 20 years of Acta Crystallographica Section F!🎉🥳 Join us by contributing to our celebratory collection of articles, which is now open for submissions! Find out more at journals.iucr.org/special_issu...

A new type of article for Acta F: Protocols The purpose of this new category is to provide to the reader procedures that are sufficiently detailed so other researchers can reproduce the described workflow. Find out more in 'Show me how it's done: introducing Protocols': doi.org/10.1107/S205...

We're celebrating 20 years of Acta Crystallographica Section F!🎉🥳 Join us by contributing to our celebratory collection of articles, which is now open for submissions! Find out more at journals.iucr.org/special_issu...

A crystal structure of PLpro exhibits Ubl2 in two distinct conformations: the well characterized closed state, where Ubl2 is positioned near the PLpro domain, and an as yet uncharacterized open state #SARSCoV2 #PapainLikeProteases #Ubl2Domain doi.org/10.1107/S205...

A new crystal structure of ApePCNA1 and the newly solved structure of the ApePCNA2 homotrimer allowed modeling of the ApePCNA1–ApePCNA2–ApePCNA3 heterotrimer #AeropyrumPernix #DNAReplication #ProliferatingCellNuclearAntigens doi.org/10.1107/S205...

The crystal structure of GABA aminotransferase from A. thaliana and structural comparison supports the conclusion that it belongs to the class III aminotransferase family #ArabidopsisThaliana #CrystalStructure #GABAAminotransferase doi.org/10.1107/S205...

The molecular interactions of the insomnia drug vorenorexant with human orexin receptors were analysed through crystal structure analysis and docking simulations #Vornorexant #OrexinReceptors #CrystalStructure doi.org/10.1107/S205...

This structure of macrophage migration inhibitory factor is a contribution to our cross-journal Early Career Scientists in Structural Science special issue. Find out more at journals.iucr.org/early-career... #SSGCID #MacrophageMigrationInhibitoryFactor doi.org/10.1107/S205...

July issue is out now! journals.iucr.org/f/issues/202... This month's cover star is the type VI secretion system adaptor protein Tla3 from Pseudomonas aeruginosa strain UCBPPPA14. Find out more about this structure at doi.org/10.1107/S205...

📢 Applications open! Join the 9th International School on Biological Crystallization in Granada Gain hands-on experience + learn from leading experts in macromolecular crystallization. Don’t miss this opportunity! 🔗 www.isbcgranada.org #Crystallography #StructuralBiology #ScienceTraining

The structure of spectrin-like repeat 24 of human dystrophin is in excellent agreement with the AlphaFold model, supporting AlphaFold modeling of all 24 dystrophin spectrin-like repeats #Dystrophin #SpectrinLikeRepeat #DuchenneMuscularDystrophy doi.org/10.1107/S205...

The structure of a complex between human RAGE ectodomain and S100A6 variant Y84C provides a possible model for S100 covalent homodimerization that is fully compatible with RAGE binding #S100Proteins #Cysteines #CovalentHomodimerization doi.org/10.1107/S205...

The first structure of a cold-active arginase reveals a modest reduction in structural rigidity associated with cold adaptation #ColdActiveEnzymes #Arginases #XRayCrystallography doi.org/10.1107/S205...

New paper out in @actacrystf.iucr.org! We benchmarked cryoSPARC for amyloid helical reconstruction: ease of use over RELION, but amyloid-specific development still needed & software interoperability remains a problem. @landerlab.bsky.social Kelly Lab @scripps.edu doi.org/10.1107/S205...

Acetylation-mimicking K214Q and K216Q variant structures of Escherichia coli glucokinase showed that acetylation mimics impaired activity and produced domain shifts #Glucokinases #LysineAcetylation #AcetylationMimickingMutations doi.org/10.1107/S205...

Structural and enzymatic characterization of an Asp49 phospolipase A2 (PLA2) from Lachesis muta expands our understanding of structure–function relationships among viperid PLA2s #SnakeVenomPhospholipaseA2 #LachesisMuta #ProteinOligomer doi.org/10.1107/S205...

The June issue is out! journals.iucr.org/f/issues/202... The cover illustration - Plasmodium vivax macrophage migration inhibitory factor - is from an article forming part of our Early Career Scientists in Structural Science special issue. Find out more and submit: journals.iucr.org/early-career...

Thank you to my coauthor Stefania Hanau and to @actacrystf.iucr.org 6590 downloads is very satisfying. Thank you to all those readers.

In our latest episode of Interviews With Authors, we discussed PentatricoPeptide Repeat (PPR) proteins, their roles in RNA binding, use in protein design and other things, and I'd like to share some highlights. What are PPR proteins and why do they matter, you may ask? Anuradha has the answers:

It was lovely to chat with Anu and @charliebondxray.bsky.social about PPR proteins, their RNA binding modes and awesome modularity. Please watch, give the IUCr a thumbs up, and add some comments when you have a minute! What would you like to see in these interviews?

In the latest in our Interviews With Authors series, the Acta F Section Editors talk to Anuradha Pullakhandam and Charlie Bond about their review on 'Structures of pentatricopeptide repeat proteins'. Read the article at journals.iucr.org/f/issues/202... www.youtube.com/watch?v=nYcF...

New issue is out! journals.iucr.org/f/issues/202... On the cover: the atomic structure of human dystrophin spectrin-like repeat 24 determined at 2.14 Å resolution journals.iucr.org/f/issues/202...

New issue is out! journals.iucr.org/f/issues/202... On the cover: the atomic structure of human dystrophin spectrin-like repeat 24 determined at 2.14 Å resolution journals.iucr.org/f/issues/202...

Modifying the bacterial strain led to cleaner bacterial expression of a synthetic BRIL antibody that facilitates protein structure determination via both crystallography and cryo-EM #CryoEM #XRayCrystallography #BRIL doi.org/10.1107/S205...

High-resolution structures of α-bCA2 with sulfonamide derivatives provides experimentally supported information defining their binding mode and supporting inhibitor optimization #CarbonicAnhydraseII #SulfonamideInhibitors #XRayCrystallography doi.org/10.1107/S205...

An acylated GLP-1/GIP analogue peptide forms a uniquely porous spiral crystal structure composed of clockwise-ascending monomers in a square pattern #GLP1 #Peptides #Crystallization doi.org/10.1107/S205...

A comprehensive survey catalogues all pentatricopeptide repeat protein crystal structures, analysing their crystallographic properties, structural features and contributions to understanding function #PentatricopeptideRepeatProteins #RNARecognition doi.org/10.1107/S205...

Our April issue is out now! journals.iucr.org/f/issues/202... Crystallization was investigated as a preparative strategy for a GLP-1/GIP lipopeptide and led to what is believed to be the first reported crystal structure of an unbound GLP-1/GIP analogue doi.org/10.1107/S205...

Abe, Fukui and Ogura: Crystal structure of PM1Pgh, a poly-γ-glutamate hydrolase from Bacillus phage PM1 #PolyGlutamateHydrolases #Pgh #BacteriophagePM1 @NaraWomensUniv...#IUCr https://journals.iucr.org/paper?S2053230X26002189

The structure of endo-β-N-acetylglucosaminidase was determined at 1.8 Å resolution, revealing a unique spatial arrangement of domain IV, which is suggested to contribute to substrate recognition #EndoNAcetylglucosaminidases #GH85 #ComplexNGlycans doi.org/10.1107/S205...

The structure of a chimeric efflux pump was determined by cryoEM under apo and kanamycin-supplemented conditions and adopted symmetric-like and asymmetric conformations #PseudomonasAeruginosa #DrugEffluxTransporters #MembraneProteins doi.org/10.1107/S205...

Cooper and Isom: Optimized bacterial expression of a synthetic BRIL antibody #CryoEM #XRayCrystallography #BRIL @UniofOxford@UniofOxford...#IUCr https://journals.iucr.org/paper?S2053230X26001548

MBP fusion is an effective and generalizable approach to enable crystallographic studies of engineered binding proteins that are otherwise difficult to crystallize #Monobody #Aggregation #MaltoseBindingProtein doi.org/10.1107/S205...

M​. Y. Stoliarskaia et al.: X-ray analysis of complexes of carbonic anhydrase II with 1,3-oxazole-containing sulfonamide derivatives elucidates the structural basis for their exceptionally high inhibitory potency ... #IUCr https://journals.iucr.org/paper?S2053230X26002128