John Steele

John Steele

@jfcsteele.bsky.social

Structural biologist and biochemist with an interest in synthetic biology for green chemistry. Unapologetic geek passionate about science and biotechnology. Postdoc at Edinburgh University- all views are my own. He/Him.

294 followers518 following47 posts

John Steele reposted

John Steele reposted

New paper from our lab, led by @jfcsteele.bsky.social and @biowacko.bsky.social! Here we screen natural enzyme variants with genetic and process optimizations to produce an efficient system to convert lignin-derived waste (guaiacol) into nylon precursor, adipic acid. pubs.acs.org/doi/full/10....

pubs.acs.orgHeterologous Rieske Nonheme Iron Monooxygenases Enable Efficient Microbial Conversion of Lignin Guaiacol to Adipic AcidAdipic acid (1,6-hexanedioic acid) is a key building block for nylon-6,6, a widely used polymer in the global chemical industry. Current industrial production relies on petrochemical feedstocks and nitric acid oxidation of cyclohexane/cyclohexanol mixtures, releasing nitrous oxide, a potent greenhouse gas. Biotechnological routes offer sustainable alternatives but have been limited by low yields or reliance on multistrain systems. Here we report a one-pot, single-strain microbial process for the efficient conversion of guaiacol─a lignin-derived aromatic─into adipic acid. By integrating heterologous Rieske nonheme iron monooxygenases from Cupriavidus necator N-1 with systematic process optimizations in engineered Escherichia coli, we achieve near-quantitative conversion with 97% yield and titers of 1.5 g/L in aqueous, lab-scale reactions. This work demonstrates a novel and efficient strategy for lignin valorization through engineered microbial synthesis, providing a new sustainable and scalable route to adipic acid.

It's hard to describe how valuable CASP is to the structural biology & structure prediction field. The ever increasing number of prediction algorithms means independent assessment of their accuracy is vital. Please contribute interesting/tricky structural data if you can!

Torsten Schwede@torstenschwede.bsky.social

Is #AI hitting a plateau in structure prediction? Help us find out at CASP17! 🧪🧬 Calling for Targets: Immune Complexes, protein - ligand complexes, RNA/DNA, conformational ensembles, membrane proteins, viral origins, and large complexes. The Rule of Thumb: If AF3 can’t model it, we want it.

It feels cheesy when people say this but it really was a privilege to be part of this work. Our recent paper (led by the fantastic Ben Royer @swallacelab.bsky.social) shows how engineered Ecoli can turn the main product of PET plastic breakdown it into an anti-Parkinsons drug (L-DOPA) rdcu.be/e84ZO

rdcu.beMicrobial upcycling of plastic waste to levodopaNature Sustainability - The production of high-value chemicals can involve energy-intensive processes, necessitating sustainable production strategies. Here the authors present a circular...

For me this is a really important piece of work- beyond the fundamental understandings applicable to other researchers (use of transporters, identification of intermediate inhibition, product degradation route) it highlights the potential for PET plastic as a valuable precursor.

Recycling rates for PET are shockingly low. The more we show the value in collecting & treating "waste" PET, the more we encourage wholehearted participation. If Incentives aren't strong enough to recycle PET for rPET at $2 per kg, what about if your product is $50K per kg? What else can we make?

John Steele reposted

Great news that @ukri.org is substantially supporting @daphnejacksontr.bsky.social - an impactful scheme to re-start careers and access the skills of those who have taken career breaks.

Daphne Jackson Trust@daphnejacksontr.bsky.social

The Government will more than double support for the Daphne Jackson Trust from £1.7m to £4m per year, which will help to unlock the talent of many more researchers whose careers have been paused. Find out more in our joint-statement with @ukri.org daphnejackson.org/2026/03/ukri... #ResearchReturners

John Steele reposted

Why were people obsessed with Nikola Tesla’s (lack of) sex life? What happened when work took me to a country where being gay can mean life imprisonment? Why did a queer astronaut take that secret to her grave? Some of the many Qs I covered in my #LGBTHistoryMonth talk to @aktcharity.bsky.social

John Steele reposted

Please spread the word: the Structural Studies Division @mrclmb.bsky.social is looking for a new tenure-track, independent group leader with an exciting plan in any area of Structural (Molecular & Cell) biology, in discovery biology and/or methods development. 🥳 mrc.tal.net/vx/mobile-0/...

mrc.tal.netResearch Group Leader Tenure Track - Structural Studies - LMB 2775 - Medical Research CouncilLocation: Cambridge. Vacancy: Research Group Leader Tenure Track - Structural Studies - LMB 2775. Closing Date: 16/03/2026, 23:55

There's a lot of discussion whether economic growth & carbon emissions can be decoupled. Some critics say countries (e.g UK) reduce emissions while growing by offshoring pollution (i.e. to China). This is a fantastic rebuttal- China continues to grow economically with reduced CO2 emissions.

Simon Evans@drsimevans.carbonbrief.org

NEW ANALYSIS: China's CO2 has now been 'flat or falling' for 21 months * Down in 2025 * Still below Mar 2024 * Clean energy wave a key factor If this is China's peak (TBC) it's the climate story of the century so far… www.carbonbrief.org/...

John Steele reposted

Applications now open for Summer Studentships 2026! Successful applicants will be: 🌿In receipt of £504.38 per week for a studentship period of up to 6 weeks 🌿Invited to write a blog for the BiPS website 🌿Invited to attend and present at the next BiPS Conference Apply here: bit.ly/4qf2ZDG

@kurzgesagt.org have some fantastic videos. This could be a great opportunity and comes at a time when having accurate and trustworthy information is more vital than ever!

Elisa Granato@prokaryota.bsky.social

JOB ALERT! 📢 For the scientists who love science communication: we’re hiring at @kurzgesagt.org !🌍 Come join our team, the vibes are impeccable. ✍️ Scriptwriter (Remote) kurzgesagt.org/careers/scri... 🔍 Fact-Checker & Researcher (Munich/Berlin/Remote) kurzgesagt.org/careers/seni...

John Steele reposted

John Steele reposted

Interested in parasitology? Looking to do a PhD? Cone join me and @geordieparasit.bsky.social in Edinburgh as we try and work out how human parasites get their vitamins.

The Geordie Parasitologist@geordieparasit.bsky.social

🎓 Funded #PhD studentships in pathogen biology and parasitology *Cell and Molecular Biology*; *Host-pathogen interactions* Part of the EastBio Doctoral Landscape Award Based in the Edinburgh area of Scotland PhD 1: bit.ly/Host-pathoge... PhD 2: bit.ly/Parasite-sca... @uoe-eid.bsky.social

John Steele reposted

The Wallace Lab is expanding in 2026, with five new Postdoctoral Research Associate positions now open. If you're passionate about engineering biology for sustainability, we'd love to hear from you! 🧬 ♻️ Deadline: 12th November 2025, details below ⬇️

John Steele reposted

Very excited to announce that our collaborative manifesto for 🌱 #PlantScience #Education has now been published! Educators from >10 countries and 30 institutions have contributed to it and we are incredibly proud of the final output. Here is a short thread 🧵1/4 doi.org/10.1002/ppp3...

doi.orgA manifesto for plant science educationPlants provide oxygen, food, shelter, medicines and environmental services, without which human society could not exist. Tackling pressing and global challenges requires well-trained plant scientists....

John Steele reposted

Replying to @johninnescentre.bsky.social

Shortlisted candidates will be invited to give a seminar at the Fellows Conference, which will be held on 10 March 2026. Candidates who win Fellowships will be offered a Tenure Track Group Leader position from the outset, initially for 5 years. Find out more: www.jic.ac.uk/training-car...

jic.ac.ukIndependent Research Fellowships Leading to Tenured Faculty Positions | John Innes CentreThe John Innes Centre (JIC), is a world leading centre of excellence in plant and microbial sciences based on the Norwich Research Park, UK. We are inviting applications from outstanding researchers…

John Steele reposted

If you can't see sidechains, can you really call the resolution 3.3 Å..? 👀 And if you can't see sidechains, how do you know the helical symmetry is right..?

cryoEM papers@cryoempapers.bsky.social

Structural basis of Pseudomonas biofilm-forming functional amyloid FapC formation pubmed.ncbi.nlm.nih.gov/40991694/ #cryoEM

The proliferation of tools to generate predictive protein structures just underscores how important CASP is. There is no better way to allow unbiased & fair comparisons between tools! predictioncenter.org/index.cgi Such a shame NIH didn't renew funding

predictioncenter.orgHome - Prediction Center
Stephen Turner@stephenturner.us

Apple is entering the protein folding arena. SimpleFold: Folding Proteins is Simpler than You Think arxiv.org/abs/2509.18480 🧬🖥️🧪 github.com/apple/ml-sim...

John Steele reposted

John Steele reposted

I supervise an excellent postdoc who's looking for a postdoc or associate position in < 1 yr. Highly skilled in chem biol and biochem (peptide synthesis, native chem ligation, bacterial expression, purification, intein-based splicing, crystallography, interaction assays). Contact me if interested.

John Steele reposted

Food for thought here for anyone not working at TRUE atomic resolution…

Structural Biology@actacrystd.iucr.org

Andreas Ehler et al.: A high-resolution data set of fatty acid-binding protein structures. III. Unexpectedly high occurrence of wrong ligands #StructureBasedDrugDesign #FattyAcidBindingProteins #LigandBinding doi.org/10.1107/S205... #IUCr

John Steele reposted

If this is true, it’s abysmally shortsighted for #NIH. The new tools nurtured by the long series of #CASP challenges probably contribute far more in one year to the scientific and industrial economies than the entire funds that have supported it.

Science Magazine@science.org

Exclusive: An international scientific competition widely credited with spurring the development of artificial intelligence for biology appears to be on its deathbed. scim.ag/44ukS90

John Steele reposted

Interested in trying out life as a scientific #editor? Based in the UK? Background in #PlantScience? The @plosbiology.org crew is expanding (again!), so a place in our lovely team might be for you...

Nonia Pariente@npariente.bsky.social

I am recruiting - if you have a background in plant mol cell bio, a keen interest in sustainability and planetary health and are looking to move away from the bench, this may be for you! Have a 👀 and please reach out if Qs 1yr FTC, remote in the UK job-boards.eu.greenhouse.io/plos/jobs/46...

John Steele reposted

Would you like to write for New Scientist? We always work with freelancers, but occasionally I put out a call like this for new writers. I'm looking for 300-700 word news stories about new discoveries. Don't pitch us press releases or the big journals, we have those covered. Pay starts at 51p/word