The EMBO Journal

The EMBO Journal

@embojournal.org

Non-for-profit publication of research in molecular, cell & organismal biology - as part of @embopress.org Fast-fair-transparent / Open Access / Open Science

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How do cells know when to begin anaphase? @iaincheeseman.bsky.social et al find that Mad2 inhibition, Cdc20 phosphorylation, and Cdc20 levels each brake this transition – releasing these pathways together collapses mitosis into near immediate exit & mis-segregation link.springer.com/article/10.1...

link.springer.comDifferential roles for the spindle assembly checkpoint in error surveillance and mitotic timing - The EMBO JournalSpindle assembly checkpoint (SAC) components have been proposed to act as both a surveillance mechanism monitoring mitotic fidelity and as a timer that ensures sufficient time for chromosome alignment...
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Francis Barr and coworkers describe a new principle for phosphatase substrate recognition, where disordered regions of phosphatase regulatory subunits mimic kinase-activating subunits, & are themselves differentially regulated by PLK1 @tomogolgier.bsky.social et al link.springer.com/article/10.1...

link.springer.comActivator mimicry by PP6 regulatory subunits directs Aurora A dephosphorylation in mitosis - The EMBO JournalAurora A kinase is a conserved regulator of mitotic spindle assembly whose activity must be precisely balanced to ensure genome stability. Its activation at centrosomes and spindle microtubules is pro...
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Surprising distinction of RNA:DNA hybrid populations in live human cells: Vincent Vanoosthuyse, Emiliano Ricci et al show that rapid delivery of ready-made bacterial RNase H eliminates replication-impeding RNA:DNA hybrids, but not those giving rise to DRIP signals link.springer.com/article/10.1...

link.springer.comThe rapid delivery of RNase H variants reveals distinct classes of RNA:DNA hybrids - The EMBO JournalMultiple lines of evidence suggest that RNase H1-sensitive RNA:DNA hybrids could disrupt the stability and restart of stalled replication forks. The prolonged over-expression of RNase H1 is the most p...

The EMBO Journal reposted

Jogender Singh
@jogender-singh.bsky.social

Pathogens may evade recognition, but the damage they cause can give them away. Our review in @embojournal.org explores damage-driven immune surveillance in C. elegans and the lessons it offers for understanding immunity across species. link.springer.com/article/10.1...

link.springer.comImmune surveillance of cellular damage: lessons from Caenorhabditis elegans - The EMBO JournalInnate immune systems must detect infection rapidly and reliably, yet pathogens can often evade direct recognition by host pattern-recognition receptors. An alternative and increasingly appreciated st...
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Santosh Chauhan and collaborators identify another RNA-binding E3 ubiquitin ligase in innate immune sensing: DZIP3 couples viral RNA detection to RIG-I-MAVS activation link.springer.com/article/10.1...

link.springer.comDZIP3 is a viral RNA co-sensor for RIG-I that promotes type-I interferon responses - The EMBO JournalRNA-binding E3 ubiquitin ligases have the ability to link RNA regulation to the protein ubiquitination machinery, thereby influencing diverse biological processes. Here, we identify the RNA-binding E3...
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Saber H Saber, Frederic Meunier et asl provide new perspective on lipid-fuelled synaptic plasticity: integrating membrane turnover, β-oxidation, and protein lipidation link.springer.com/article/10.1...

link.springer.comLipid-fuelled synaptic plasticity: integrating membrane turnover, β-oxidation, and protein lipidation - The EMBO JournalSynaptic plasticity requires the coordinated regulation of membrane remodelling, energy production, and protein turnover. However, how these processes are integrated at the molecular level remains unc...

The EMBO Journal reposted

Tomoaki Sobajima
@tomogolgier.bsky.social

Our latest work from the Barr lab in @oxfordbiochemistry.bsky.social is now out in @embojournal.org Activator mimicry by PP6 regulatory subunits directs Aurora A dephosphorylation in mitosis | EMBO Journal #cellcycle #mitosis link.springer.com/article/10.1...

link.springer.comActivator mimicry by PP6 regulatory subunits directs Aurora A dephosphorylation in mitosis - The EMBO JournalAurora A kinase is a conserved regulator of mitotic spindle assembly whose activity must be precisely balanced to ensure genome stability. Its activation at centrosomes and spindle microtubules is pro...
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Do nuclear speckle (NS) components dynamically redistribute between speckles to meet local physiological demands? Andrew Belmont and colleagues show dynamic multiphase connections link NS & enable coordinated, targeted transport of macromolecules within the nucleus link.springer.com/article/10.1...

link.springer.comNon-random inter-speckle material transfer mediated by dynamic multiphase interchromatin connections - The EMBO JournalNuclear speckles (NS) enhance the expression of NS-associated genes, possibly by elevating local levels of factors involved in multiple steps of gene expression. While dozens of large NS are distribut...
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The receptor TM9SF3 controls Golgiphagy, but how is it itself regulated? Yixian Cui & colleagues show that under stress, it is stabilized by USP28-mediated deubiquitination, leading to maintenance of glycosylation patterns and thereby promoting breast tumor growth link.springer.com/article/10.1...

link.springer.comA USP28-TM9SF3-Golgiphagy axis maintains glycosylation homeostasis and promotes breast cancer progression - The EMBO JournalSelective autophagy of the Golgi apparatus (Golgiphagy) is essential for Golgi quality control, yet its regulation and physiological functions remain poorly understood. Here, we identify the deubiquit...
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Pierre Falson, @vincentchaptal.bsky.social et al present cryo-EM structures of Cdr1, the main ABC transporter involved in drug resistance in pathogenic Candida–revealing how it accommodates its substrates & defining the ATP-driven conformational cycle of this pump link.springer.com/article/10.1...

link.springer.comATP-driven dynamics and itraconazole binding of the drug efflux pump Cdr1 from Candida glabrata - The EMBO JournalAzole resistance in Candida species is often caused by the overexpression of Cdr1. Despite its clinical relevance, the structural basis for its ATP-driven efflux pump function remains elusive. We pres...
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Kinetic and functional analyses identify the distinct pathways for recycling of resident Golgi proteins regulated by key Golgi-associated tethers Benjamin Glick and coworkers link.springer.com/article/10.1...

link.springer.comGolgi-associated tethers capture vesicles from specific membrane recycling pathways - The EMBO JournalDuring maturation of a Golgi cisterna, a series of vesicular transport pathways recycle resident Golgi proteins. Recycling vesicles are captured by Golgi-associated tethers. Here, we examined three of...
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Dan Bergstralh, @lovegrovelab.bsky.social et al develop a computational model exploring contributions of astral microtubule pushing & cortical pulling forces to spindle positioning & dynamics across diverse mitotic geometries - with validation in fly & fish cells link.springer.com/article/10.1...

link.springer.comMicrotubule pushing and cortical pulling cooperate to orient and position the mitotic spindle - The EMBO JournalWhile regulation of spindle positioning and orientation is known to be mediated by cortical pulling forces generated by dynein on astral microtubules, the contribution of microtubule-based pushing for...
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How does DNA remain confined to dying cells despite its fragmentation? @mfdspicer.bsky.social @rcollepardo.bsky.social @gerlichlab.bsky.social et al show histone deacetylation compacts apoptotic chromatin, preventing it from entering apoptotic extracellular vesicles link.springer.com/article/10.1...

link.springer.comElectrostatic control of chromatin compaction safeguards against apoptotic DNA release - The EMBO JournalApoptosis involves extensive intracellular reorganisation to facilitate the clearance of dying cells. A key step in this process is the destruction of the genome through fragmentation by caspase-activ...
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Epithelial wound healing has mainly been analyzed at the apical plane of the epithelium – @yanlanmao.bsky.social and colleagues now reveal a contractile system that connects the apical and basal cell surfaces at the wound edge for efficient wound closure link.springer.com/article/10.1...

link.springer.comThree-dimensional mechanical cooperativity optimises epithelial wound healing - The EMBO JournalEpithelial tissues serve as critical physical barriers that protect organs, making their efficient repair essential upon damage. To achieve this, tissues must quickly react, forcing cells to rearrange...
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mRNA processing links to tumorigenesis: @agatakstepien.bsky.social , Deepshika Pulimamidi, @stoplab.bsky.social show shortened cleavage-to-termination distances in colorectal cancer cells, correlating with increased gene expression & dependence on CPSF73 and PCF11 link.springer.com/article/10.1...

link.springer.comProximity of pre-mRNA 3′ end processing and transcription termination is associated with enhanced gene expression - The EMBO JournalTwo key factors required for pre-mRNA 3′ end processing, CPSF73 and PCF11, are linked to poor cancer prognosis, yet their oncogenic roles remain unclear due to their dual function in transcription ter...
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What are the origins and differentiation trajectories of atypical B-cells occurring across infections? @chrissundling.bsky.social reveal parallel path from distinct precursor to a shared late phenotype link.springer.com/article/10.1...

link.springer.comExpression patterns of CD24 and CD27 resolve the origins and gradual differentiation of CD11c+ atypical memory B-cells after malaria infection - The EMBO JournalAtypical B-cells (ABCs) are observed across infections, yet their ontogeny and differentiation remain unclear. Using high-dimensional flow cytometry, single-cell transcriptomics, V(D)J sequencing, and...
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Collective cell invasion: @yuritakahashi.bsky.social, @bensteventon.bsky.social et al establish EMT initiation as an upstream inducer of BMP pathway activation during vertebrate development link.springer.com/article/10.1...

link.springer.comReciprocal coupling between EMT and BMP morphogen signalling drives collective cell invasion - The EMBO JournalDuring collective cell invasion, epithelial-to-mesenchymal transition (EMT) and morphogen signalling-mediated cell fate specification are traditionally viewed as a linear cascade: morphogens drive cel...
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New resource article: @floriangrebien.bsky.social and colleagues use bicon-seq to identify lncRNA Gas5 as a critical modulator of oncogenic condensates in fusion protein-driven leukemia link.springer.com/article/10.1...

link.springer.comUncovering essential RNA components of fusion oncoprotein condensates using bicon-seq - The EMBO JournalFusion oncoproteins drive aggressive leukemias by mediating aberrant gene expression within specialized biomolecular condensates that contain various biomolecules. While RNA is known to influence cond...
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How does Salmonella reorganize the Golgi network to establish vacuoles serving as its intracellular replicative niche? Ana Eulalio and colleagues show a non-canonical role of upregulated Connexin-43 expression, due to infection-induced downregulation of a microRNA link.springer.com/article/10.1...

link.springer.comThe miR-744-5p/connexin 43 axis modulates Salmonella-containing vacuole association with the Golgi network - The EMBO JournalMicroRNAs are increasingly recognised as important regulators of host–pathogen interactions. Using a large-scale functional screening approach, we identified miR-744-5p as a strong inhibitor of Salmon...
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Timothy Weil, @timesaunders.bsky.social and coworkers reveal quantitative spatial temporal dynamics of translation of a morphogen vivo, leading to a modified synthesis-diffusion-degradation model for bicoid gradient formation link.springer.com/article/10.1...

link.springer.comDynamics of bicoid mRNA localisation and translation dictate morphogen gradient formation - The EMBO JournalThe transcription factor Bicoid (Bcd) protein guides early Drosophila patterning and is the best-characterised morphogen. The source of the morphogen, bcd mRNA, is maternally deposited during oogenesi...
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How do cells ensure completion of DNA synthesis after under-licensing of replication origins undetected by S-phase checkpoints? Schwob, Talarek et al show that CDK phosphorylation of DNA Pol𝛂 promotes recombination-dependent completion of replication in mitosis link.springer.com/article/10.1...

link.springer.comSwitch from canonical to recombination-dependent DNA synthesis by M-CDK phosphorylation of DNA Polα - The EMBO JournalCells preserve genome stability while occasionally generating genetic diversity through mutations and chromosomal rearrangements, but how these opposing outcomes are balanced remains unclear. Because ...

The EMBO Journal reposted

Yanlan Mao
@yanlanmao.bsky.social

Latest paper from our lab is now out in @embojournal.org! If you want to know how 3D cell shape changes promote #woundhealing, and see a 3D vertex model that allows #scutoids to form, read this article: link.springer.com/article/10.1... Many thanks and congrats to all co-authors!

link.springer.comThree-dimensional mechanical cooperativity optimises epithelial wound healing - The EMBO JournalEpithelial tissues serve as critical physical barriers that protect organs, making their efficient repair essential upon damage. To achieve this, tissues must quickly react, forcing cells to rearrange...
The EMBO Journal
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Why does endogenous, but not exogenous leptin remain effective in restraining feeding in obesity? @tunepers.bsky.social, Martin G Myers and collaborators identify a set of CNS neurons controlling AgRP neuron sensitivity to orexigenic stimuli link.springer.com/article/10.1...

link.springer.comLeptin-sensitive hypothalamic Lepr+/Glp1r+ neurons limit hyperphagia and weight gain in diet-induced obesity - The EMBO JournalDespite loss of responsiveness to exogenous leptin in obesity, endogenous leptin continues to restrain feeding, although the neural substrates that remain sensitive and mediate this effect remain unkn...
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How about stress-induced ribosome hibernation in archaea? @diorgeps.bsky.social, @archaeon-alex.bsky.social, Joey Davis et al identify conserved archaeal factor AHA with evolutionary link to bacterial hibernation-promoting factor HPF & eukaryotic energy sensor AMPKγ link.springer.com/article/10.1...

link.springer.comA conserved archaeal protein with evolutionary links to bacterial ribosome hibernation and eukaryotic energy sensing - The EMBO JournalRibosome hibernation helps cells survive stress by reversibly silencing translation and preserving ribosomal complexes. Although well characterized in bacteria and eukaryotes, archaeal hibernation rem...
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Phage therapy approaches depend on phage suppression of host bacterial physiology - and P. aeruginosa phage PaoP5 has a protein, Ped1, that does this in a dual manner: via DNA degradation and replication inhibition Haihua Liang, Tietao Wang and collaborators link.springer.com/article/10.1...

link.springer.comA dual-action bacteriophage protein Ped1 degrades DNA and blocks replication in Pseudomonas aeruginosa - The EMBO JournalTo fully harness phage therapy against escalating antimicrobial resistance, it is imperative to elucidate the fundamental mechanisms by which viruses systematically dismantle host physiology. In this ...
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Why is #replication initiation factor Mcm10 essential for replicative helicase activation in yeast but not animals? @labiblab.bsky.social, @yyxia.bsky.social et al now find similar roles for worm & human homologs, masked by partial redundancy with SLD-2/RECQL4 link.springer.com/article/10.1...

link.springer.comMCM10 and SLD-2/RECQL4 jointly activate the CMG helicase during metazoan DNA replication initiation - The EMBO JournalEukaryotic cells regulate the assembly and activation of the essential DNA helicase at the heart of the chromosome replication machinery, to ensure that the chromosomes are copied just once per cell c...
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Another #RefereedPreprint ℅ @reviewcommons.org : Fumiyo Ikeda 池田 史代 and coworkers show that LUBAC subunit HOIL-1 restrains linear ubiquitination to maintain dynamic properties of p62 condensates and promote aggregate clearance link.springer.com/article/10.1...

link.springer.comExcess Met1-linked ubiquitination leads to solid aggregate formation - The EMBO JournalThe ubiquitin ligase HOIL-1 regulates the formation of Met1-linked (linear) ubiquitin chains through its coordination with the E3 ligase HOIP within the Linear Ubiquitin Chain Assembly Complex (LUBAC)...
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Bacteria have evolved #ubiquitin regulatory enzymes are distinct in sequence & structure from their eukaryotic counterparts - a new screening workflow established by @jnpruneda.bsky.social and collaborators allows their identification from complex bacterial samples link.springer.com/article/10.1...

link.springer.comA functional screen for bacterial ubiquitin regulation identifies an unusual Pseudomonas aeruginosa E3 ligase - The EMBO JournalBacterial pathogens have adapted secreted effector proteins that hijack host ubiquitination to gain control over host responses. These effectors can exhibit, for example, E3 ligase or deubiquitinase a...
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New insight into host-parasite interactions involved in Toxoplasma invasion: Sebastian Lourido and coworkers find the parasite microneme MIC1/4/6 complex to cluster host N-glycans into membrane microdomains to promote rhoptry discharge link.springer.com/article/10.1...

link.springer.comClustering of host N-glycans by the microneme MIC1/4/6 complex licenses Toxoplasma rhoptry discharge - The EMBO JournalTo initiate the process of invasion, apicomplexan parasites must discharge the contents of specialized organelles called rhoptries into host cells. This process requires prior recognition and binding ...
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Does it matter which route a cell takes towards whole-genome duplication? Simon Gemble, Renata Basto et al show that only mitotic slippage leads to softening of the nucleus, making it more susceptible to deformation through the microtubule cytoskeleton link.springer.com/article/10.1...

link.springer.comWhole genome duplication through mitotic slippage causes nuclear instability - The EMBO JournalWhole-genome duplication (WGD), leading to polyploidy can arise in physiological and pathological contexts. WGD can occur via non-canonical cell cycles such as mitotic slippage, cytokinesis failure or...
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How doe BRCA1 suppress Group 1 genomic tandem duplications? @ralphscully.bsky.social show that Brca1 coiled-coil mutant mouse models remain competent for suppression of tandem duplications despite being defective for homologous recombination link.springer.com/article/10.1...

link.springer.comThe BRCA1 coiled-coil domain is dispensable for suppression of tandem duplications and tolerance of FANCM loss - The EMBO JournalBRCA1-linked cancers contain abundant ~10 kb ‘Group 1’ tandem duplications (TDs). Group 1 TDs form at a Tus/Ter replication-fork barrier in DNA-end resection-defective mouse embryonic stem (mES) cells...
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What defines limits & regulation of epigenetic inheritance? Florian Steiner et al show altered genomic distribution of histone modification H3K27me3 can be transmitted across C. elegans generations through successive SET-32 & MES-4 action that alters its deposition link.springer.com/article/10.1...

link.springer.comTransgenerational inheritance of altered H3K27me3 in wild-type Caenorhabditis elegans - The EMBO JournalEpigenetic inheritance provides mutation-free mechanisms for phenotypic adaptation, including modifications to DNA or DNA-associated proteins that create patterns of heritable gene regulation. However...
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Wanqi Liang and collaborators show that rice ZEP1, a transverse-filament protein of the synaptonemal complex, coordinates anti-crossover activities and crossover pathways to promote proper intermediate processing and faithful recombination during #meiosis link.springer.com/article/10.1...

link.springer.comZEP1 orchestrates template choice and crossover pathways to ensure meiotic genome integrity in rice - The EMBO JournalMeiotic crossovers (COs) are tightly regulated to ensure chromosome segregation while limiting aberrant recombination. Transverse filament (TF) proteins of the synaptonemal complex (SC) regulate class...
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Akiyasu Iwase, Hiroki Kurihara et al show that loss of Hox-dependent regional identity enables pharyngeal neural crest cells to acquire new fates during mammalian heart development link.springer.com/article/10.1...

link.springer.comHox/Meis-dependent gene-regulatory transition underlies cardiopharyngeal neural crest diversification - The EMBO JournalNeural crest cells (NCCs) are multipotent migratory cells essential for cardiac development, yet the lineage trajectories and gene regulatory networks underlying their differentiation in the cardiopha...

The EMBO Journal reposted

www.MitoWorld.org
@mitoworld.bsky.social

How do cells decide whether to store energy as fat or mobilize it? #Mitochondria play a pivotal role! Learn more from Dr. Shirihai on his @embojournal.org paper on the Mitoblog: mitoworld.org/a-calcium-sw... #UMDF #mitoscientists #Brownadiposetissue #adiposetissue #mitochondrialmetabolism

mitoworld.orgA Calcium Switch for Fat: How Mitochondria Balance Lipid Storage and Utilization - MitoWorldCells face a continual metabolic decision: should fatty acids be stored for later, or mobilized and burned for energy? Mitochondria sit at the heart of both processes. Although they are best known for...
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Biochemical diversity of tubulin: Carsten Janke, Zdenek Lansky and collaborators screen 46 microtubule-associated proteins to reveal their distinct affinities to tubulin isotypes and posttranslational modifications link.springer.com/article/10.1...

link.springer.comMicrotubule posttranslational modifications provide unique recognition patterns for associated proteins - The EMBO JournalMicrotubules are key components of the eukaryotic cytoskeleton involved in vital functions in virtually every cell. Among the emerging molecular mechanisms to adapt microtubules to their diverse funct...
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Loss of oxygen-sensing chromatin regulator KDM6A drives a pseudo-hyperoxic metabolic state through two pathways: via increased mitochondria synthesis, and via increased mitochondria trafficking from stromal cells to cancer cells Giovanni Tonon and collaborators link.springer.com/article/10.1...

link.springer.comKDM6A loss enhances oxidative phosphorylation uncovering tissue-level convergent evolution - The EMBO JournalThe tumor suppressor KDM6A/UTX, a histone demethylase and a 2-oxoglutarate-dependent dioxygenase, is frequently lost in many cancer types. We show that KDM6A loss pervasively activates oxidative phosp...