Devine lab

Devine lab

@devinelab.bsky.social

Devine lab at @crick.ac.uk We study how mitochondria regulate neuronal synapses and how that regulation goes wrong in diseases that affect the brain Posts by lab members | Views are own

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Devine lab
@devinelab.bsky.social

Here we describe step-by-step how to visualise dopaminergic release in acute brain slices, using the fluorescent false neurotransmitter 200 (FFN200). This protocol can be used with other FFNs, and explains steps from imaging🔬 to data analysis✅

Devine lab@devinelab.bsky.social

We are excited to share a new paper from our lab presenting a protocol developed by Patrick Cottilli during his PhD placement at Sulzer lab in Columbia University, NY www.cell.com/star-protoco...

cell.comProtocol for fluorescent false neurotransmitter live imaging of dopamine release dynamics from individual synapses in acute brain slicesStriatal dopaminergic (DA) axons have many en passant boutons with synaptic vesicles, but only a fraction display exocytosis. Here, we present a protocol for live imaging of murine DA axons using fluo...
Devine lab
@devinelab.bsky.social

We are excited to share a new paper from our lab presenting a protocol developed by Patrick Cottilli during his PhD placement at Sulzer lab in Columbia University, NY www.cell.com/star-protoco...

cell.comProtocol for fluorescent false neurotransmitter live imaging of dopamine release dynamics from individual synapses in acute brain slicesStriatal dopaminergic (DA) axons have many en passant boutons with synaptic vesicles, but only a fraction display exocytosis. Here, we present a protocol for live imaging of murine DA axons using fluo...
Devine lab
@devinelab.bsky.social

Check out the first review from the Mitochondrial Neurobiology lab on... lysosomes! Learn about the non-canonical roles of lysosomes in neurons from @jonnyspencer.bsky.social @ysudarikova.bsky.social: www.cell.com/trends/neuro...

cell.comNon-canonical roles of lysosomes in neuronsNeurons are highly polarised and compartmentalised cells with organelles that are specialised to support their spatial and functional demands. This includes lysosomes, which are single-membrane-bound ...
Devine lab
@devinelab.bsky.social

Congratulations to Dr. Patrick Cottilli who has successfully defended his PhD viva yesterday!!🎉 This is a huge milestone for our lab - our first-ever PhD graduate!⭐ So proud of your hard work, creativity, and perseverance throughout these 4 years. Here's to the amazing science ahead!🔬

Devine lab
@devinelab.bsky.social

We report that MitoTracker, a dye commonly used to label mitochondria, transfers from astrocytes to neurons independently of mitochondrial transfer ⚠️ This should prompt an essential reevaluation of the literature concerning mitochondrial transfer that has been observed using mitochondrial dyes 📖

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